Anti-ALOX15B Polyclonal Antibody (HT104014)

Name: Anti-ALOX15B Polyclonal Antibody
Brand: abinScience
SKU: HT104014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HT104014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ALOX15B (Ala2-Gly121).

Target

15-lipoxygenase 2, 15-LOX-B, 15-LOX-2, Linoleate 13-lipoxygenase 15-LOb, Arachidonate 15-lipoxygenase B, Polyunsaturated fatty acid lipoxygenase ALOX15B, ALOX15B, Arachidonate 15-lipoxygenase type II

Purification

Purified by antigen affinity column.

Accession

O15296

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Polyunsaturated fatty acid lipoxygenase ALOX15B is a ~75 kDa protein. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist. Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates.

1. Kilty, I. et al. (1999) European journal of biochemistry 266, 83-93. PMID: 10542053

2. Jisaka, M. et al. (2000) The Journal of biological chemistry 275, 1287-93. PMID: 10625675

3. Bhatia, B. et al. (2003) The Journal of biological chemistry 278, 25091-100. PMID: 12704195

4. Prusakiewicz, JJ. et al. (2007) Archives of biochemistry and biophysics 464, 260-8. PMID: 17493578

5. Turman, MV. et al. (2008) Biochemistry 47, 3917-25. PMID: 18311922

6. Horn, T. et al. (2013) Redox biology 1, 566-77. PMID: 24282679

7. Kobe, MJ. et al. (2014) The Journal of biological chemistry 289, 8562-9. PMID: 24497644

8. Perry, SC. et al. (2020) Journal of lipid research 61, 1087-1103. PMID: 32404334

9. Brash, AR. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 6148-52. PMID: 9177185

Note

For research use only.

Images

References

Datasheet

Anti-ALOX15B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-ALOX15B Polyclonal Antibody (HT104014)

Overview

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Datasheet

SDS

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