Human BECN1 Recombinant Protein (N-His) (HC377022)

Name: Human BECN1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC377022
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HC377022
설명	Recombinant Human BECN1 Protein, N-His (HC377022) expressed in E. coli, spanning Thr141-Lys450. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	Q14457
단백질 길이	Thr141-Lys450
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	38.79 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, GT197
배경	Beclin-1 (BECN1) is a ~51 kDa protein. Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. 1. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871 2. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617 3. Margariti, A. et al. (2013) The Journal of biological chemistry 288, 859-72. PMID: 23184933 4. Xia, P. et al. (2013) The EMBO journal 32, 2685-96. PMID: 23974797 5. Xu, C. et al. (2014) Autophagy 10, 2239-50. PMID: 25484083 6. Ashkenazi, A. et al. (2017) Nature 545, 108-111. PMID: 28445460 7. Wyatt, S. et al. (2023) Biochemistry 62, 2934-2951. PMID: 37776275 8. Sagona, AP. et al. (2010) Nature cell biology 12, 362-71. PMID: 20208530 9. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123 10. Liu, K. et al. (2016) The Journal of cell biology 212, 181-98. PMID: 26783301
참고사항	For research use only.