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| 카탈로그 번호 | HP018012 |
|---|---|
| 설명 |
Recombinant Human BHLHE41 Protein, N-His (HP018012) expressed in E. coli, spanning Asp40-Leu174. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9C0J9 |
| 단백질 길이 | Asp40-Leu174 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 17.93 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | bHLHe41, hDEC2, DEC2, bHLHb3, Enhancer-of-split and hairy-related protein 1, Class E basic helix-loop-helix protein 41, Class B basic helix-loop-helix protein 3, BHLHB3, SHARP1, SHARP-1, Differentially expressed in chondrocytes protein 2, BHLHE41 |
| 배경 | Class E basic helix-loop-helix protein 41 (BHLHE41) is a ~50 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. 1. Garriga-Canut, M. et al. (2001) The Journal of biological chemistry 276, 14821-8. PMID: 11278948 2. Kawamoto, T. et al. (2004) Biochemical and biophysical research communications 313, 117-24. PMID: 14672706 3. Li, Y. et al. (2004) The Biochemical journal 382, 895-904. PMID: 15193144 4. Sato, F. et al. (2004) European journal of biochemistry 271, 4409-19. PMID: 15560782 5. Nakashima, A. et al. (2008) Molecular and cellular biology 28, 4080-92. PMID: 18411297 6. Cho, Y. et al. (2009) Molecular pharmacology 76, 1360-9. PMID: 19786558 7. Pellegrino, R. et al. (2014) Sleep 37, 1327-36. PMID: 25083013 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human BHLHE41 Protein, N-His
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