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| 카탈로그 번호 | HV669012 |
|---|---|
| 설명 |
Recombinant Human BIRC6 Protein, N-His (HV669012) expressed in E. coli, spanning Arg4576-Leu4857. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9NR09 |
| 단백질 길이 | Arg4576-Leu4857 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 34.13 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | KIAA1289, BIRC6, BRUCE, RING-type E3 ubiquitin transferase BIRC6, BIR repeat-containing ubiquitin-conjugating enzyme, APOLLON, Baculoviral IAP repeat-containing protein 6, Ubiquitin-conjugating BIR domain enzyme apollon |
| 배경 | Dual E2 ubiquitin-conjugating enzyme/E3 ubiquitin-protein ligase BIRC6 is a ~530 kDa protein. Anti-apoptotic protein known as inhibitor of apoptosis (IAP) which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Unlike most IAPs, does not contain a RING domain and it is not a RING-type E3 ligase. Instead acts as a dual E2/E3 enzyme that combines ubiquitin conjugating (E2) and ubiquitin ligase (E3) activities in a single polypeptide. Ubiquitination is mediated by a non-canonical E1 ubiquitin activating enzyme UBA6. Ubiquitinates CASP3, CASP7 and CASP9 and inhibits their caspase activity; also ubiquitinates their procaspases but to a weaker extent. 1. Qiu, XB. et al. (2004) The EMBO journal 23, 800-10. PMID: 14765125 2. Bartke, T. et al. (2004) Molecular cell 14, 801-11. PMID: 15200957 3. Pohl, C. et al. (2008) Cell 132, 832-45. PMID: 18329369 4. Hunkeler, M. et al. (2023) Science (New York, N.Y.) 379, 1105-1111. PMID: 36758104 5. Ehrmann, JF. et al. (2023) Science (New York, N.Y.) 379, 1117-1123. PMID: 36758105 6. Dietz, L. et al. (2023) Science (New York, N.Y.) 379, 1112-1117. PMID: 36758106 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human BIRC6 Protein, N-His
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