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| 카탈로그 번호 | HB192012 |
|---|---|
| 설명 |
Recombinant Human BRD2 Protein, N-His (HB192012) expressed in E. coli, spanning Arg74-Gln182. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P25440 |
| 단백질 길이 | Arg74-Gln182 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 15.31 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Really interesting new gene 3 protein, KIAA9001, O27.1.1, BRD2, RING3, Bromodomain-containing protein 2 |
| 배경 | Bromodomain-containing protein 2 (BRD2) is a ~88 kDa protein. Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation. Also recognizes and binds acetylated non-histone proteins, such as STAT3. Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin. 1. Nakamura, Y. et al. (2007) The Journal of biological chemistry 282, 4193-201. PMID: 17148447 2. Huang, H. et al. (2007) BMC structural biology 7, 57. PMID: 17848202 3. LeRoy, G. et al. (2008) Molecular cell 30, 51-60. PMID: 18406326 4. Umehara, T. et al. (2010) The Journal of biological chemistry 285, 7610-8. PMID: 20048151 5. Umehara, T. et al. (2010) FEBS letters 584, 3901-8. PMID: 20709061 6. Filippakopoulos, P. et al. (2010) Nature 468, 1067-73. PMID: 20871596 7. Cheung, KL. et al. (2017) Molecular cell 65, 1068-1080.e5. PMID: 28262505 8. Xie, L. et al. (2022) Nature genetics 54, 481-491. PMID: 35410381 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human BRD2 Protein, N-His
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