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| 카탈로그 번호 | HW474012 |
|---|---|
| 설명 |
Recombinant Human CASP4 Protein, N-His (HW474012) expressed in E. coli, spanning Asn78-Glu265. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P49662 |
| 단백질 길이 | Asn78-Glu265 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 23.5 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Mih1, ICH2, CASP-4, ICE and Ced-3 homolog 2, CASP4, Protease TX, Caspase-4, ICE(rel)-II, ICH-2 |
| 배경 | Caspase-4 (CASP4) is a ~43 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B. In the non-canonical inflammasome, CASP4 is activated by direct binding to the lipid A moiety of LPS without the need of an upstream sensor. 1. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034 2. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003 3. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412 4. Li, Z. et al. (2021) Nature 599, 290-295. PMID: 34671164 5. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519 6. Devant, P. et al. (2023) Nature 624, 451-459. PMID: 37993712 7. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714 8. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580 10. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CASP4 Protein, N-His
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