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Anti-CASP4 Polyclonal Antibody (HW474014)

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Overview
Catalog No.HW474014
Description
Anti-CASP4 Polyclonal Antibody (HW474014) is a rabbit polyclonal antibody detecting CASP4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CASP4 (Asn78-Glu265).
Target Mih1, ICH2, CASP-4, ICE and Ced-3 homolog 2, CASP4, Protease TX, Caspase-4, ICE(rel)-II, ICH-2
Purification Purified by antigen affinity column.
Accession P49662
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Caspase-4 (CASP4) is a ~43 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B. In the non-canonical inflammasome, CASP4 is activated by direct binding to the lipid A moiety of LPS without the need of an upstream sensor.

1. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034
2. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003
3. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412
4. Li, Z. et al. (2021) Nature 599, 290-295. PMID: 34671164
5. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519
6. Devant, P. et al. (2023) Nature 624, 451-459. PMID: 37993712
7. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714
8. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580
10. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-CASP4 Polyclonal Antibody

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