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| 카탈로그 번호 | HW613012 |
|---|---|
| 설명 |
Recombinant Human CASR Protein, N-His (HW613012) expressed in E. coli, spanning Tyr63-Ser531. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P41180 |
| 단백질 길이 | Tyr63-Ser531 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 55.11 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Parathyroid cell calcium-sensing receptor 1, CaR, Extracellular calcium-sensing receptor, PCAR1, GPRC2A, PCaR1, CASR, hCasR, CaSR |
| 배경 | Extracellular calcium-sensing receptor (CASR) is a ~120 kDa protein. G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis. Senses fluctuations in the circulating calcium concentration: activated by elevated circulating calcium, leading to decreased parathyroid hormone (PTH) secretion in parathyroid glands. In kidneys, acts as a key regulator of renal tubular calcium resorption. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G-proteins) and modulates the activity of downstream effectors. CASR is coupled with different G(q)/G(11), G(i)/G(o)- or G(s)-classes of G-proteins depending on the context. 1. Tõke, J. et al. (2007) Clinical endocrinology 67, 385-92. PMID: 17555508 2. Lietman, SA. et al. (2009) The Journal of clinical endocrinology and metabolism 94, 4372-9. PMID: 19789209 3. Al-Salameh, A. et al. (2011) European journal of endocrinology 165, 359-63. PMID: 21566075 4. Grant, MP. et al. (2011) Science signaling 4, ra78. PMID: 22114145 5. Guarnieri, V. et al. (2012) Molecular genetics and metabolism 107, 548-52. PMID: 22789683 6. Nakamura, A. et al. (2013) The Journal of clinical endocrinology and metabolism 98, E1692-701. PMID: 23966241 7. Stratta, P. et al. (2014) Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 29, 1902-9. PMID: 25104082 8. Mastromatteo, E. et al. (2014) BMC endocrine disorders 14, 81. PMID: 25292184 9. Baran, N. et al. (2015) Molecular and cellular endocrinology 407, 18-25. PMID: 25766501 10. Kim, ES. et al. (2016) Journal of bone and mineral metabolism 34, 662-667. PMID: 26386835 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CASR Protein, N-His
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