Anti-Human CASR Polyclonal Antibody (HW613014)

Name: Anti-Human CASR Polyclonal Antibody
Brand: abinScience
SKU: HW613014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW613014

Description

Anti-Human CASR Polyclonal Antibody (HW613014) is a rabbit polyclonal antibody detecting CASR in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CASR (Tyr63-Ser531).

Target

Parathyroid cell calcium-sensing receptor 1, CaR, Extracellular calcium-sensing receptor, PCAR1, GPRC2A, PCaR1, CASR, hCasR, CaSR

Purification

Purified by antigen affinity column.

Accession

P41180

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Extracellular calcium-sensing receptor (CASR) is a ~120 kDa protein. G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis. Senses fluctuations in the circulating calcium concentration: activated by elevated circulating calcium, leading to decreased parathyroid hormone (PTH) secretion in parathyroid glands. In kidneys, acts as a key regulator of renal tubular calcium resorption. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G-proteins) and modulates the activity of downstream effectors. CASR is coupled with different G(q)/G(11), G(i)/G(o)- or G(s)-classes of G-proteins depending on the context.

1. Tõke, J. et al. (2007) Clinical endocrinology 67, 385-92. PMID: 17555508

2. Lietman, SA. et al. (2009) The Journal of clinical endocrinology and metabolism 94, 4372-9. PMID: 19789209

3. Al-Salameh, A. et al. (2011) European journal of endocrinology 165, 359-63. PMID: 21566075

4. Grant, MP. et al. (2011) Science signaling 4, ra78. PMID: 22114145

5. Guarnieri, V. et al. (2012) Molecular genetics and metabolism 107, 548-52. PMID: 22789683

6. Nakamura, A. et al. (2013) The Journal of clinical endocrinology and metabolism 98, E1692-701. PMID: 23966241

7. Stratta, P. et al. (2014) Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 29, 1902-9. PMID: 25104082

8. Mastromatteo, E. et al. (2014) BMC endocrine disorders 14, 81. PMID: 25292184

9. Baran, N. et al. (2015) Molecular and cellular endocrinology 407, 18-25. PMID: 25766501

10. Kim, ES. et al. (2016) Journal of bone and mineral metabolism 34, 662-667. PMID: 26386835

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human CASR polyclonal antibody at 1ug/mL on recombinant Human CASR (Catalog No. HW613012).

References

Datasheet