ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
55.11 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Extracellular calcium-sensing receptor (CASR) is a ~120 kDa protein. G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis. Senses fluctuations in the circulating calcium concentration: activated by elevated circulating calcium, leading to decreased parathyroid hormone (PTH) secretion in parathyroid glands. In kidneys, acts as a key regulator of renal tubular calcium resorption. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G-proteins) and modulates the activity of downstream effectors. CASR is coupled with different G(q)/G(11), G(i)/G(o)- or G(s)-classes of G-proteins depending on the context.
1. Tõke, J. et al. (2007) Clinical endocrinology 67, 385-92. PMID: 17555508
2. Lietman, SA. et al. (2009) The Journal of clinical endocrinology and metabolism 94, 4372-9. PMID: 19789209
3. Al-Salameh, A. et al. (2011) European journal of endocrinology 165, 359-63. PMID: 21566075
5. Guarnieri, V. et al. (2012) Molecular genetics and metabolism 107, 548-52. PMID: 22789683
6. Nakamura, A. et al. (2013) The Journal of clinical endocrinology and metabolism 98, E1692-701. PMID: 23966241
7. Stratta, P. et al. (2014) Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 29, 1902-9. PMID: 25104082
8. Mastromatteo, E. et al. (2014) BMC endocrine disorders 14, 81. PMID: 25292184
9. Baran, N. et al. (2015) Molecular and cellular endocrinology 407, 18-25. PMID: 25766501
10. Kim, ES. et al. (2016) Journal of bone and mineral metabolism 34, 662-667. PMID: 26386835
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CASR Protein, N-His
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