Human CD317/BST2 Recombinant Protein (C-His) (HC341011)

Name: Human CD317/BST2 Recombinant Protein (C-His)
Brand: abinScience
SKU: HC341011
Price: 298 USD
Availability: InStock

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50ug
100ug
1mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HC341011
설명	Recombinant Human CD317/BST2 Protein, C-His (HC341011) expressed in Mammalian Cells, spanning Asn49-Ser160. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	Mammalian cells
등록번호	Q10589
단백질 길이	Asn49-Ser160
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	15.86 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	Tetherin, Bone marrow stromal antigen 2, BST2, BST-2, CD317, HM1.24 antigen
배경	Bone marrow stromal antigen 2 (CD317/BST2) is a ~19 kDa protein. IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, paramyxoviridae: nipah virus, and coronaviridae: SARS-CoV. 1. Neil, SJ. et al. (2008) Nature 451, 425-30. PMID: 18200009 2. Van Damme, N. et al. (2008) Cell host & microbe 3, 245-52. PMID: 18342597 3. Jouvenet, N. et al. (2009) Journal of virology 83, 1837-44. PMID: 19036818 4. Perez-Caballero, D. et al. (2009) Cell 139, 499-511. PMID: 19879838 5. Miyakawa, K. et al. (2009) PLoS pathogens 5, e1000700. PMID: 20019814 6. Hinz, A. et al. (2010) Cell host & microbe 7, 314-323. PMID: 20399176 7. Pardieu, C. et al. (2010) PLoS pathogens 6, e1000843. PMID: 20419159 8. Yang, H. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18428-32. PMID: 20940320 9. Xu, F. et al. (2011) Virology journal 8, 198. PMID: 21529378 10. Dafa-Berger, A. et al. (2012) Virology 428, 98-111. PMID: 22520941
참고사항	For research use only.

이미지

SDS-PAGE

SDS-PAGE for Recombinant Human CD317/BST2 Protein, C-His
Bioactivity

Detects Human CD317/BST2 Antibody (HM1.24) in indirect ELISAs.
Western blot

Detected by Anti-CD317/BST2 monoclonal antibody (Catalog No: HC341107)