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Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His) (HV126011)

Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)
Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)
Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)
Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)
Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)
Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)
가격(USD):
사양:
  • 50ug
  • 100ug
  • 1mg
수량:
문의하기
  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HV126011
설명
Recombinant Human CD85k/LILRB4/ILT3 Protein, C-His (HV126011) expressed in Mammalian Cells, spanning Met1-Glu259. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템Mammalian cells
등록번호Q8NHJ6
단백질 길이Met1-Glu259
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Homo sapiens (Human)
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
예측 분자량 29.47 kDa
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름CD85 antigen-like family member K, LIR-5, Leukocyte immunoglobulin-like receptor 5, ILT3, ILT-3, Immunoglobulin-like transcript 3, LILRB4, CD85k, Leukocyte immunoglobulin-like receptor subfamily B member 4, Monocyte inhibitory receptor HM18, LIR5
배경

Leukocyte immunoglobulin-like receptor subfamily B member 4 (CD85k/LILRB4) is a ~49 kDa protein. Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance. Receptor for FN1. Receptor for apolipoprotein APOE. Receptor for ALCAM/CD166. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

1. Chang, CC. et al. (2002) Nature immunology 3, 237-43. PMID: 11875462
2. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617
3. Deng, M. et al. (2018) Nature 562, 605-609. PMID: 30333625
4. Xu, Z. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1207-1219. PMID: 29263213
5. Cella, M. et al. (1997) The Journal of experimental medicine 185, 1743-51. PMID: 9151699
6. Lu, HK. et al. (2009) The Journal of biological chemistry 284, 34839-48. PMID: 19833736
7. Kim-Schulze, S. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 2790-8. PMID: 16493035
참고사항 For research use only.
이미지
  • Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CD85k/LILRB4/ILT3 Protein, C-His

  • Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)

    Bioactivity

    Detects Human CD85k/LILRB4 Antibody (128-3#) in indirect ELISAs.

  • Human LILRB4/CD85k/ILT3 Recombinant Protein (C-His)

    Bioactivity

    Detects IO-202 in indirect ELISAs.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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