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| 카탈로그 번호 | HW357022 |
|---|---|
| 설명 |
Recombinant Human CDK9 Protein, N-His (HW357022) expressed in E. coli, spanning Glu15-Ala340. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P50750 |
| 단백질 길이 | Glu15-Ala340 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 39.77 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | TAK, CDC2L4, C-2K, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Cyclin-dependent kinase 9, CDK9, Tat-associated kinase complex catalytic subunit |
| 배경 | Cyclin-dependent kinase 9 (CDK9) is a ~42 kDa protein. Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELFE. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. 1. Fu, TJ. et al. (1999) The Journal of biological chemistry 274, 34527-30. PMID: 10574912 2. Ivanov, D. et al. (2000) Molecular and cellular biology 20, 2970-83. PMID: 10757782 3. Kim, JB. et al. (2001) The Journal of biological chemistry 276, 12317-23. PMID: 11145967 4. Lavoie, SB. et al. (2001) Journal of molecular biology 312, 675-85. PMID: 11575923 5. Bourgeois, CF. et al. (2002) Molecular and cellular biology 22, 1079-93. PMID: 11809800 6. Lin, X. et al. (2002) The Journal of biological chemistry 277, 16873-8. PMID: 11884399 7. Fujinaga, K. et al. (2004) Molecular and cellular biology 24, 787-95. PMID: 14701750 8. Jang, MK. et al. (2005) Molecular cell 19, 523-34. PMID: 16109376 9. Yang, Z. et al. (2005) Molecular cell 19, 535-45. PMID: 16109377 10. Yu, DS. et al. (2010) EMBO reports 11, 876-82. PMID: 20930849 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CDK9 Protein, N-His
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