Anti-Human CDK9 Polyclonal Antibody (HW357024)

Name: Anti-Human CDK9 Polyclonal Antibody
Brand: abinScience
SKU: HW357024
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW357024

Description

Anti-Human CDK9 Polyclonal Antibody (HW357024) is a rabbit polyclonal antibody detecting CDK9 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Danio rerio, Human, Xenopus laevis, and Gallus gallus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CDK9 (Asp104-Phe315).

Target

CDK9

Purification

Purified by antigen affinity column.

Accession

P50750

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Cyclin-dependent kinase 9, 2.7.11.22, 2.7.11.23, C-2K, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Tat-associated kinase complex catalytic subunit, CDK9, CDC2L4, TAK

Background

Cyclin-dependent kinase 9 (CDK9) is a ~42 kDa protein. Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELFE. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g.

1. Fu, TJ. et al. (1999) The Journal of biological chemistry 274, 34527-30. PMID: 10574912

2. Ivanov, D. et al. (2000) Molecular and cellular biology 20, 2970-83. PMID: 10757782

3. Kim, JB. et al. (2001) The Journal of biological chemistry 276, 12317-23. PMID: 11145967

4. Lavoie, SB. et al. (2001) Journal of molecular biology 312, 675-85. PMID: 11575923

5. Bourgeois, CF. et al. (2002) Molecular and cellular biology 22, 1079-93. PMID: 11809800

6. Lin, X. et al. (2002) The Journal of biological chemistry 277, 16873-8. PMID: 11884399

7. Fujinaga, K. et al. (2004) Molecular and cellular biology 24, 787-95. PMID: 14701750

8. Jang, MK. et al. (2005) Molecular cell 19, 523-34. PMID: 16109376

9. Yang, Z. et al. (2005) Molecular cell 19, 535-45. PMID: 16109377

10. Yu, DS. et al. (2010) EMBO reports 11, 876-82. PMID: 20930849

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CDK9 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse lung lysate
Lane 2: Rat brain lysate
Lane 3: Rat lung lysate