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| 카탈로그 번호 | HT321012 |
|---|---|
| 설명 |
Recombinant Human CEP290 Protein, N-His (HT321012) expressed in E. coli, spanning Thr1289-Arg1416. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O15078 |
| 단백질 길이 | Thr1289-Arg1416 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 17.89 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Nephrocystin-6, KIAA0373, CEP290, Bardet-Biedl syndrome 14 protein, Tumor antigen se2-2, BBS14, NPHP6, Centrosomal protein of 290 kDa, Cancer/testis antigen 87, Cep290, CT87 |
| 배경 | Centrosomal protein of 290 kDa (CEP290) is a ~290 kDa protein. Involved in early and late steps in cilia formation. Its association with CCP110 is required for inhibition of primary cilia formation by CCP110. May play a role in early ciliogenesis in the disappearance of centriolar satellites and in the transition of primary ciliar vesicles (PCVs) to capped ciliary vesicles (CCVs). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1. Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes. 1. Tsang, WY. et al. (2008) Developmental cell 15, 187-97. PMID: 18694559 2. Kobayashi, T. et al. (2014) The Journal of cell biology 204, 215-29. PMID: 24421332 3. Frank, V. et al. (2008) Human mutation 29, 45-52. PMID: 17705300 4. Barbelanne, M. et al. (2015) Human molecular genetics 24, 2185-200. PMID: 25552655 5. Sayer, JA. et al. (2006) Nature genetics 38, 674-81. PMID: 16682973 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CEP290 Protein, N-His
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