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| 카탈로그 번호 | HT043012 |
|---|---|
| 설명 |
Recombinant Human CYP27B1 Protein, N-His (HT043012) expressed in E. coli, spanning Asp256-Arg508. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O15528 |
| 단백질 길이 | Asp256-Arg508 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 30.39 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, CYP27B, CYP27B1, Cytochrome P450C1 alpha, Calcidiol 1-monooxygenase, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP1ALPHA, Cytochrome P450 subfamily XXVIIB polypeptide 1, 25-OHD-1 alpha-hydroxylase, VD3 1A hydroxylase, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial |
| 배경 | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) is a ~56 kDa protein. A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. 1. Sawada, N. et al. (1999) European journal of biochemistry 265, 950-6. PMID: 10518789 2. Kitanaka, S. et al. (1999) The Journal of clinical endocrinology and metabolism 84, 4111-7. PMID: 10566658 3. Wang, X. et al. (2002) The Journal of clinical endocrinology and metabolism 87, 2424-30. PMID: 12050193 4. Tang, EKY. et al. (2012) The FEBS journal 279, 3749-3761. PMID: 22862690 5. Kitanaka, S. et al. (1998) The New England journal of medicine 338, 653-61. PMID: 9486994 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CYP27B1 Protein, N-His
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