Anti-CYP27B1 Polyclonal Antibody (HT043014)

Name: Anti-CYP27B1 Polyclonal Antibody
Brand: abinScience
SKU: HT043014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HT043014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYP27B1 (Asp256-Arg508).

Target

25-hydroxyvitamin D(3) 1-alpha-hydroxylase, CYP27B, CYP27B1, Cytochrome P450C1 alpha, Calcidiol 1-monooxygenase, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP1ALPHA, Cytochrome P450 subfamily XXVIIB polypeptide 1, 25-OHD-1 alpha-hydroxylase, VD3 1A hydroxylase, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Purification

Purified by antigen affinity column.

Accession

O15528

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) is a ~56 kDa protein. A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2.

1. Sawada, N. et al. (1999) European journal of biochemistry 265, 950-6. PMID: 10518789

2. Kitanaka, S. et al. (1999) The Journal of clinical endocrinology and metabolism 84, 4111-7. PMID: 10566658

3. Wang, X. et al. (2002) The Journal of clinical endocrinology and metabolism 87, 2424-30. PMID: 12050193

4. Tang, EKY. et al. (2012) The FEBS journal 279, 3749-3761. PMID: 22862690

5. Kitanaka, S. et al. (1998) The New England journal of medicine 338, 653-61. PMID: 9486994

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CYP27B1 polyclonal antibody at 1ug/mL on recombinant CYP27B1 (Catalog No. HT043012).

References

Datasheet

Anti-CYP27B1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CYP27B1 Polyclonal Antibody (HT043014)

Overview

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References

Datasheet

SDS

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