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| 카탈로그 번호 | HP031012 |
|---|---|
| 설명 |
Recombinant Human DGCR8 Protein, N-His (HP031012) expressed in E. coli, spanning Asp436-Lys752. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q8WYQ5 |
| 단백질 길이 | Asp436-Lys752 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 39.12 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | DGCRK6, Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12 |
| 배경 | Microprocessor complex subunit DGCR8 is a ~86 kDa protein. Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. 1. Nguyen, TA. et al. (2015) Cell 161, 1374-87. PMID: 26027739 2. Kwon, SC. et al. (2016) Cell 164, 81-90. PMID: 26748718 3. Gregory, RI. et al. (2004) Nature 432, 235-40. PMID: 15531877 4. Han, J. et al. (2004) Genes & development 18, 3016-27. PMID: 15574589 5. Landthaler, M. et al. (2004) Current biology : CB 14, 2162-7. PMID: 15589161 6. Han, J. et al. (2006) Cell 125, 887-901. PMID: 16751099 7. Pauley, KM. et al. (2006) EMBO reports 7, 904-10. PMID: 16906129 8. Yeom, KH. et al. (2006) Nucleic acids research 34, 4622-9. PMID: 16963499 9. Faller, M. et al. (2007) Nature structural & molecular biology 14, 23-9. PMID: 17159994 10. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human DGCR8 Protein, N-His
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