Anti-Human DGCR8 Polyclonal Antibody (HP031014)

Name: Anti-Human DGCR8 Polyclonal Antibody
Brand: abinScience
SKU: HP031014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP031014

Description

Anti-Human DGCR8 Polyclonal Antibody (HP031014) is a rabbit polyclonal antibody detecting DGCR8 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human DGCR8 (Asp436-Lys752).

Target

DGCRK6, Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12

Purification

Purified by antigen affinity column.

Accession

Q8WYQ5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Microprocessor complex subunit DGCR8 is a ~86 kDa protein. Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding.

1. Nguyen, TA. et al. (2015) Cell 161, 1374-87. PMID: 26027739

2. Kwon, SC. et al. (2016) Cell 164, 81-90. PMID: 26748718

3. Gregory, RI. et al. (2004) Nature 432, 235-40. PMID: 15531877

4. Han, J. et al. (2004) Genes & development 18, 3016-27. PMID: 15574589

5. Landthaler, M. et al. (2004) Current biology : CB 14, 2162-7. PMID: 15589161

6. Han, J. et al. (2006) Cell 125, 887-901. PMID: 16751099

7. Pauley, KM. et al. (2006) EMBO reports 7, 904-10. PMID: 16906129

8. Yeom, KH. et al. (2006) Nucleic acids research 34, 4622-9. PMID: 16963499

9. Faller, M. et al. (2007) Nature structural & molecular biology 14, 23-9. PMID: 17159994

10. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998

Note

For research use only.

Images

References

Datasheet

Anti-Human DGCR8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human DGCR8 Polyclonal Antibody (HP031014)

Overview

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Datasheet

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