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| 카탈로그 번호 | HT429012 |
|---|---|
| 설명 |
Recombinant Human DRP1/DNM1L Protein, N-His (HT429012) expressed in E. coli, spanning Met1-Arg516. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O00429 |
| 단백질 길이 | Met1-Arg516 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Dynamin family member proline-rich carboxyl-terminal domain less, DVLP, Dynamin-like protein IV, Dynamin-related protein 1, Dynamin-like protein, DLP1, Dymple, Dynamin-1-like protein, HdynIV, Dnm1p/Vps1p-like protein, DRP1, DNM1L, Dynamin-like protein 4 |
| 배경 | Dynamin-1-like protein (DRP1/DNM1L) is a ~81 kDa protein. Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane. Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner. 1. Smirnova, E. et al. (2001) Molecular biology of the cell 12, 2245-56. PMID: 11514614 2. Koch, A. et al. (2003) The Journal of biological chemistry 278, 8597-605. PMID: 12499366 3. Taguchi, N. et al. (2007) The Journal of biological chemistry 282, 11521-9. PMID: 17301055 4. Waterham, HR. et al. (2007) The New England journal of medicine 356, 1736-41. PMID: 17460227 5. Chang, CR. et al. (2007) The Journal of biological chemistry 282, 21583-7. PMID: 17553808 6. Han, XJ. et al. (2008) The Journal of cell biology 182, 573-85. PMID: 18695047 7. Cereghetti, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15803-8. PMID: 18838687 8. Cho, DH. et al. (2009) Science (New York, N.Y.) 324, 102-5. PMID: 19342591 9. Zunino, R. et al. (2009) The Journal of biological chemistry 284, 17783-95. PMID: 19411255 10. Figueroa-Romero, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 3917-27. PMID: 19638400 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human DRP1/DNM1L Protein, N-His
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