Anti-DRP1/DNM1L Polyclonal Antibody (HT429014)

Name: Anti-DRP1/DNM1L Polyclonal Antibody
Brand: abinScience
SKU: HT429014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HT429014

Description

Anti-DRP1/DNM1L Polyclonal Antibody (HT429014) is a rabbit polyclonal antibody detecting DNM1L in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DRP1/DNM1L (Met1-Arg516).

Target

Dynamin family member proline-rich carboxyl-terminal domain less, DVLP, Dynamin-like protein IV, Dynamin-related protein 1, Dynamin-like protein, DLP1, Dymple, Dynamin-1-like protein, HdynIV, Dnm1p/Vps1p-like protein, DRP1, DNM1L, Dynamin-like protein 4

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O00429

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dynamin-1-like protein (DRP1/DNM1L) is a ~81 kDa protein. Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane. Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner.

1. Smirnova, E. et al. (2001) Molecular biology of the cell 12, 2245-56. PMID: 11514614

2. Koch, A. et al. (2003) The Journal of biological chemistry 278, 8597-605. PMID: 12499366

3. Taguchi, N. et al. (2007) The Journal of biological chemistry 282, 11521-9. PMID: 17301055

4. Waterham, HR. et al. (2007) The New England journal of medicine 356, 1736-41. PMID: 17460227

5. Chang, CR. et al. (2007) The Journal of biological chemistry 282, 21583-7. PMID: 17553808

6. Han, XJ. et al. (2008) The Journal of cell biology 182, 573-85. PMID: 18695047

7. Cereghetti, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15803-8. PMID: 18838687

8. Cho, DH. et al. (2009) Science (New York, N.Y.) 324, 102-5. PMID: 19342591

9. Zunino, R. et al. (2009) The Journal of biological chemistry 284, 17783-95. PMID: 19411255

10. Figueroa-Romero, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 3917-27. PMID: 19638400

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DRP1/DNM1L polyclonal antibody at 1ug/mL on recombinant DRP1/DNM1L (Catalog No. HT429012).

References

Datasheet

Anti-DRP1/DNM1L Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-DRP1/DNM1L Polyclonal Antibody (HT429014)

Overview

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References

Datasheet

SDS

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