ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Dynamin family member proline-rich carboxyl-terminal domain less, DVLP, Dynamin-like protein IV, Dynamin-related protein 1, Dynamin-like protein, DLP1, Dymple, Dynamin-1-like protein, HdynIV, Dnm1p/Vps1p-like protein, DRP1, DNM1L, Dynamin-like protein 4
Background
Dynamin-1-like protein (DRP1/DNM1L) is a ~81 kDa protein. Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane. Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner.
1. Smirnova, E. et al. (2001) Molecular biology of the cell 12, 2245-56. PMID: 11514614
2. Koch, A. et al. (2003) The Journal of biological chemistry 278, 8597-605. PMID: 12499366
3. Taguchi, N. et al. (2007) The Journal of biological chemistry 282, 11521-9. PMID: 17301055
4. Waterham, HR. et al. (2007) The New England journal of medicine 356, 1736-41. PMID: 17460227
5. Chang, CR. et al. (2007) The Journal of biological chemistry 282, 21583-7. PMID: 17553808
6. Han, XJ. et al. (2008) The Journal of cell biology 182, 573-85. PMID: 18695047
7. Cereghetti, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15803-8. PMID: 18838687
8. Cho, DH. et al. (2009) Science (New York, N.Y.) 324, 102-5. PMID: 19342591
9. Zunino, R. et al. (2009) The Journal of biological chemistry 284, 17783-95. PMID: 19411255
10. Figueroa-Romero, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 3917-27. PMID: 19638400
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human DRP1/DNM1L Protein, N-His
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