中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HV499022 |
|---|---|
| 설명 |
Recombinant Human ERI1 Protein, N-His (HV499022) expressed in E. coli, spanning Pro64-Ser329. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q8IV48 |
| 단백질 길이 | Pro64-Ser329 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 33.44 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | 3'-5' exonuclease ERI1, 3'-5' exoribonuclease 1, 3'EXO, EC:3.1.13.1, ERI1, Eri-1 homolog, HEXO, Histone mRNA 3'-end-specific exoribonuclease, Histone mRNA 3'-exonuclease 1, Protein 3'hExo, THEX1 |
| 배경 | 3'-5' exoribonuclease 1 is a ~40 kDa protein. RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient 3'-end histone mRNA exonuclease activity and degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Required for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. 1. Dominski, Z. et al. (2003) Molecular cell 12, 295-305. PMID: 14536070 2. Yang, XC. et al. (2006) The Journal of biological chemistry 281, 30447-54. PMID: 16912046 3. Kupsco, JM. et al. (2006) RNA (New York, N.Y.) 12, 2103-17. PMID: 17135487 4. Guo, L. et al. (2023) American journal of human genetics 110, 1068-1085. PMID: 37352860 5. Kennedy, S. et al. (2004) Nature 427, 645-9. PMID: 14961122 |
| 참고사항 | For research use only |
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
