Human ERI1 Recombinant Protein (N-His) (HV499012)

Name: Human ERI1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV499012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV499012
Description	Recombinant Human ERI1 Protein, N-His (HV499012) expressed in E. coli, spanning Met1-Lys249. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q8IV48
Protein length	Met1-Lys249
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.92 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	HEXO, Histone mRNA 3'-end-specific exoribonuclease, Protein 3'hExo, Eri-1 homolog, 3'-5' exonuclease ERI1, Histone mRNA 3'-exonuclease 1, 3'-5' exoribonuclease 1, THEX1, 3'EXO, ERI1
Background	3'-5' exoribonuclease 1 (ERI1) is a ~40 kDa protein. RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient 3'-end histone mRNA exonuclease activity and degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Required for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. 1. Dominski, Z. et al. (2003) Molecular cell 12, 295-305. PMID: 14536070 2. Yang, XC. et al. (2006) The Journal of biological chemistry 281, 30447-54. PMID: 16912046 3. Kupsco, JM. et al. (2006) RNA (New York, N.Y.) 12, 2103-17. PMID: 17135487 4. Guo, L. et al. (2023) American journal of human genetics 110, 1068-1085. PMID: 37352860 5. Kennedy, S. et al. (2004) Nature 427, 645-9. PMID: 14961122
Note	For research use only.

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SDS-PAGE for Recombinant Human ERI1 Protein, N-His

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Datasheet

Human ERI1 Recombinant Protein (N-His).pdf

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Human ERI1 Recombinant Protein (N-His) (HV499012)

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