Human NMDAR1 Recombinant Protein (N-His) (HC465022)

Name: Human NMDAR1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC465022
Price: 211 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

50ug
100ug
1mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HC465022
설명	Recombinant Human GRIN1/NMDAR1 Protein, N-His (HC465022) expressed in E. coli, spanning Arg19-Gln559. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	Q05586
단백질 길이	Arg19-Gln559
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	62.85 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	Glutamate receptor ionotropic NMDA 1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, hNR1, GRIN1, NMDAR1
배경	Glutamate receptor ionotropic, NMDA 1 is a ~105 kDa protein. Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium. Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators. 1. Hamdan, FF. et al. (2011) American journal of human genetics 88, 306-16. PMID: 21376300 2. Hackos, DH. et al. (2016) Neuron 89, 983-99. PMID: 26875626 3. Volgraf, M. et al. (2016) Journal of medicinal chemistry 59, 2760-79. PMID: 26919761 4. Chen, W. et al. (2017) Molecular pharmacology 91, 317-330. PMID: 28126851 5. Chen, W. et al. (2017) Journal of human genetics 62, 589-597. PMID: 28228639 6. Zhang, J. et al. (2023) Nature structural & molecular biology 30, 629-639. PMID: 36959261 7. Karp, SJ. et al. (1993) The Journal of biological chemistry 268, 3728-33. PMID: 7679115 8. Younkin, DP. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 2174-8. PMID: 7681588 9. Planells-Cases, R. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 5057-61. PMID: 7685113
참고사항	For research use only