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| 카탈로그 번호 | HY418012 |
|---|---|
| 설명 |
Recombinant Human HSP90AB1 Protein, N-His (HY418012) expressed in E. coli, spanning Met1-Asp724. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P08238 |
| 단백질 길이 | Met1-Asp724 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 85.42 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | HSPC2, HSP 90, HSP90B, Heat shock protein HSP 90-beta, Heat shock 84 kDa, HSPCB, HSP84, HSP90AB1, HSP 84 |
| 배경 | Heat shock protein HSP 90-beta (HSP90AB1) is a ~83 kDa protein. Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. 1. Chadli, A. et al. (2006) Molecular and cellular biology 26, 1722-30. PMID: 16478993 2. Retzlaff, M. et al. (2009) EMBO reports 10, 1147-53. PMID: 19696785 3. Pearl, LH. (2016) Biopolymers 105, 594-607. PMID: 26991466 4. Verma, S. et al. (2016) Biochimie 127, 227-40. PMID: 27295069 5. Khurana, N. et al. (2015) Frontiers in oncology 5, 100. PMID: 25973397 6. Shang, Y. et al. (2014) Biochemical and biophysical research communications 446, 387-92. PMID: 24613385 7. Didelot, C. et al. (2008) Cell death and differentiation 15, 859-66. PMID: 18239673 8. Cheng, MB. et al. (2010) Cellular signalling 22, 1206-13. PMID: 20353823 9. Zhang, M. et al. (2020) Cell 181, 637-652.e15. PMID: 32272059 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human HSP90AB1 Protein, N-His
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