中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HY426012 |
|---|---|
| 설명 |
Recombinant Human IFIT1 Protein, N-His (HY426012) expressed in E. coli, spanning Met1-Pro478. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P09914 |
| 단백질 길이 | Met1-Pro478 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 57.52 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | IFI-56K, G10P1, Interferon-induced protein with tetratricopeptide repeats 1, IFIT-1, IFNAI1, IFIT1, ISG56, IFI56, Interferon-induced 56 kDa protein, P56 |
| 배경 | Antiviral innate immune response effector IFIT1 is a ~55 kDa protein. Plays a key role in the innate immune response as part of an interferon-dependent multiprotein complex, recognizing and sequestering viral RNAs that lack host-specific 2'-O-methylation at their 5' cap. By distinguishing these RNAs from host mRNAs, inhibits their translation by competing with the translation initiation factor eIF4E. Could also prevent viral replication through its interaction with DNA replication origin-binding protein E1 of several viruses. Causes the translocation of E1 from the nucleus to the cytoplasm and can also inhibit its helicase activity in vitro. Exhibits antiviral activity against many viruses from the Flaviviridae (West Nile virus, Dengue virus, hepatitis C virus), Coronaviridae (human 229E coronavirus, SARS-CoV-2 and SARS-CoV), Poxviridae (vaccinia virus) and Togaviridae (Sindbis virus) families. 1. Pichlmair, A. et al. (2011) Nature immunology 12, 624-30. PMID: 21642987 2. Daugherty, MD. et al. (2016) eLife 5. PMID: 27240734 3. Geng, J. et al. (2024) RNA (New York, N.Y.) 30, 1292-1305. PMID: 39009378 4. Abbas, YM. et al. (2013) Nature 494, 60-4. PMID: 23334420 5. Abbas, YM. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E2106-E2115. PMID: 28251928 6. Russ, A. et al. (2022) EMBO reports 23, e55648. PMID: 36285486 7. Terenzi, F. et al. (2008) The EMBO journal 27, 3311-21. PMID: 19008854 8. Raychoudhuri, A. et al. (2011) Journal of virology 85, 12881-9. PMID: 21976647 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human IFIT1 Protein, N-His
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
