Human IL-15 Recombinant Protein (C-His) (HW541012)

Name: Human IL-15 Recombinant Protein (C-His)
Brand: abinScience
SKU: HW541012
Price: 211 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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사양:

50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HW541012
설명	Recombinant Human IL15 Protein, C-His (HW541012) expressed in E. coli, spanning Asn49-Ser162. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	P40933
단백질 길이	Asn49-Ser162
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
테스트된 응용 분야	WB
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	14.03 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	IL15, IL-15, Interleukin-15
배경	Interleukin-15 (IL15) is a ~18 kDa protein. Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines. In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection. Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5. 1. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770 2. Grabstein, KH. et al. (1994) Science (New York, N.Y.) 264, 965-8. PMID: 8178155 3. Badolato, R. et al. (1997) Blood 90, 2804-9. PMID: 9326248 4. Musso, T. et al. (1999) Blood 93, 3531-9. PMID: 10233906 5. Ring, AM. et al. (2012) Nature immunology 13, 1187-95. PMID: 23104097 6. Giri, JG. et al. (1994) The EMBO journal 13, 2822-30. PMID: 8026467 7. Johnston, JA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8705-9. PMID: 7568001
참고사항	For research use only.