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| 카탈로그 번호 | HV817032 |
|---|---|
| 설명 |
Recombinant Human IRS2 Protein, N-His (HV817032) expressed in E. coli, spanning Gly185-Arg301. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9Y4H2 |
| 단백질 길이 | Gly185-Arg301 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 15.31 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | IRS-2, IRS2, Insulin receptor substrate 2 |
| 배경 | Insulin receptor substrate 2 is a ~137 kDa protein. Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. 1. Fukushima, T. et al. (2015) Nature communications 6, 6780. PMID: 25879670 2. Geng, Y. et al. (2014) The Journal of biological chemistry 289, 11230-11241. PMID: 24616100 3. Duan, C. et al. (2004) The Journal of biological chemistry 279, 43684-91. PMID: 15316008 4. Heller, NM. et al. (2008) Science signaling 1, ra17. PMID: 19109239 5. Manohar, S. et al. (2020) Molecular & cellular proteomics : MCP 19, 1450-1467. PMID: 32554797 |
| 참고사항 | For research use only |
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