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| 카탈로그 번호 | HB572012 |
|---|---|
| 설명 |
Recombinant Human MEFV Protein, N-His (HB572012) expressed in E. coli, spanning Glu589-Asp781. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O15553 |
| 단백질 길이 | Glu589-Asp781 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 23.48 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Pyrin; Marenostrin; MEFV; MEF; TRIM20 |
| 배경 | Pyrin is a ~86 kDa protein. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production. Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection. 1. Centola, M. et al. (2000) Blood 95, 3223-31. PMID: 10807793 2. Mansfield, E. et al. (2001) Blood 98, 851-9. PMID: 11468188 3. Yu, JW. et al. (2006) Cell death and differentiation 13, 236-49. PMID: 16037825 4. Chae, JJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 9982-7. PMID: 16785446 5. Papin, S. et al. (2007) Cell death and differentiation 14, 1457-66. PMID: 17431422 6. Yu, JW. et al. (2007) Molecular cell 28, 214-27. PMID: 17964261 7. Chae, JJ. et al. (2008) Blood 112, 1794-803. PMID: 18577712 8. Waite, AL. et al. (2009) Experimental biology and medicine (Maywood, N.J.) 234, 40-52. PMID: 19109554 9. Waite, AL. et al. (2009) PloS one 4, e6147. PMID: 19584923 10. Kimura, T. et al. (2015) The Journal of cell biology 210, 973-89. PMID: 26347139 |
| 참고사항 | For research use only |
SDS-PAGE for Recombinant Human MEFV Protein, N-His
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