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| 카탈로그 번호 | HC254012 |
|---|---|
| 설명 |
Recombinant Human NEDD8 Protein, N-His-SUMO (HC254012) expressed in E. coli, spanning Leu2-Gln81. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q15843 |
| 단백질 길이 | Leu2-Gln81 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 21.28 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | NEDD-8, NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8 |
| 배경 | Ubiquitin-like protein NEDD8 is a ~9 kDa protein. Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. 1. Liakopoulos, D. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 5510-5. PMID: 10318914 2. Hori, T. et al. (1999) Oncogene 18, 6829-34. PMID: 10597293 3. Amir, RE. et al. (2002) The Journal of biological chemistry 277, 23253-9. PMID: 11953428 4. Walden, H. et al. (2003) Molecular cell 12, 1427-37. PMID: 14690597 5. Xirodimas, DP. et al. (2004) Cell 118, 83-97. PMID: 15242646 6. Osaka, F. et al. (1998) Genes & development 12, 2263-8. PMID: 9694792 7. Horn-Ghetko, D. et al. (2024) Nature structural & molecular biology 31, 1083-1094. PMID: 38605244 8. Kokic, G. et al. (2024) Nature structural & molecular biology 31, 536-547. PMID: 38316879 |
| 참고사항 | For research use only |
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