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| 카탈로그 번호 | HP884012 |
|---|---|
| 설명 |
Recombinant Human NHEJ1 Protein, N-His (HP884012) expressed in E. coli, spanning Met1-Ser127. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9H9Q4 |
| 단백질 길이 | Met1-Ser127 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 16.53 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Non-homologous end-joining factor 1, XRCC4-like factor, NHEJ1, XLF, Protein cernunnos |
| 배경 | Non-homologous end-joining factor 1 (NHEJ1) is a ~33 kDa protein. DNA repair protein involved in DNA non-homologous end joining (NHEJ); it is required for double-strand break (DSB) repair and V(D)J recombination and is also involved in telomere maintenance. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. 1. Buck, D. et al. (2006) Cell 124, 287-99. PMID: 16439204 2. Ahnesorg, P. et al. (2006) Cell 124, 301-13. PMID: 16439205 3. Lu, H. et al. (2007) The Journal of biological chemistry 282, 11155-62. PMID: 17317666 4. Tsai, CJ. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 7851-6. PMID: 17470781 5. Gu, J. et al. (2007) Nucleic acids research 35, 5755-62. PMID: 17717001 6. Andres, SN. et al. (2007) Molecular cell 28, 1093-101. PMID: 18158905 7. Yu, Y. et al. (2008) DNA repair 7, 1680-92. PMID: 18644470 8. Malivert, L. et al. (2010) The Journal of biological chemistry 285, 26475-83. PMID: 20558749 9. Roy, S. et al. (2015) Molecular and cellular biology 35, 3017-28. PMID: 26100018 10. Carrillo, J. et al. (2017) Human molecular genetics 26, 1900-1914. PMID: 28369633 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human NHEJ1 Protein, N-His
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