Anti-Human NHEJ1 Polyclonal Antibody (HP884014)

Name: Anti-Human NHEJ1 Polyclonal Antibody
Brand: abinScience
SKU: HP884014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HP884014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NHEJ1 (Met1-Ser127).

Target

Non-homologous end-joining factor 1, XRCC4-like factor, NHEJ1, XLF, Protein cernunnos

Purification

Purified by antigen affinity column.

Accession

Q9H9Q4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Non-homologous end-joining factor 1 (NHEJ1) is a ~33 kDa protein. DNA repair protein involved in DNA non-homologous end joining (NHEJ); it is required for double-strand break (DSB) repair and V(D)J recombination and is also involved in telomere maintenance. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired.

1. Buck, D. et al. (2006) Cell 124, 287-99. PMID: 16439204

2. Ahnesorg, P. et al. (2006) Cell 124, 301-13. PMID: 16439205

3. Lu, H. et al. (2007) The Journal of biological chemistry 282, 11155-62. PMID: 17317666

4. Tsai, CJ. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 7851-6. PMID: 17470781

5. Gu, J. et al. (2007) Nucleic acids research 35, 5755-62. PMID: 17717001

6. Andres, SN. et al. (2007) Molecular cell 28, 1093-101. PMID: 18158905

7. Yu, Y. et al. (2008) DNA repair 7, 1680-92. PMID: 18644470

8. Malivert, L. et al. (2010) The Journal of biological chemistry 285, 26475-83. PMID: 20558749

9. Roy, S. et al. (2015) Molecular and cellular biology 35, 3017-28. PMID: 26100018

10. Carrillo, J. et al. (2017) Human molecular genetics 26, 1900-1914. PMID: 28369633

Note

For research use only.

Images

References

Datasheet

Anti-Human NHEJ1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human NHEJ1 Polyclonal Antibody (HP884014)

Overview

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Datasheet

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