中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HD130012 |
|---|---|
| 설명 |
Recombinant Human ORF2p Protein, N-His (HD130012) expressed in E. coli, spanning Met1-Lys240. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O00370 |
| 단백질 길이 | Met1-Lys240 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 29.56 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | EC:2.7.7.49, EC:3.1.21.-, Endonuclease, LINE-1 retrotransposable element ORF2 protein, ORF2p, Reverse transcriptase |
| 배경 | LINE-1 retrotransposable element ORF2 protein is a ~149 kDa protein. Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition. 1. Thawani, A. et al. (2024) Nature 626, 186-193. PMID: 38096901 2. Baldwin, ET. et al. (2024) Nature 626, 194-206. PMID: 38096902 3. Dombroski, BA. et al. (1994) Molecular and cellular biology 14, 4485-92. PMID: 7516468 4. Sassaman, DM. et al. (1997) Nature genetics 16, 37-43. PMID: 9140393 5. Repanas, K. et al. (2007) Nucleic acids research 35, 4914-26. PMID: 17626046 6. Miller, I. et al. (2021) Nucleic acids research 49, 11350-11366. PMID: 34554261 7. Feng, Q. et al. (1996) Cell 87, 905-16. PMID: 8945517 |
| 참고사항 | For research use only |
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
