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| 카탈로그 번호 | HP333012 |
|---|---|
| 설명 |
Recombinant Human PIP5K1A Protein, N-His (HP333012) expressed in E. coli, spanning Lys62-Lys452. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q99755 |
| 단백질 길이 | Lys62-Lys452 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 47.08 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | PIP5K1-alpha, PtdIns(4)P-5-kinase 1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, PIP5KIalpha, 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha, PIP5K1A, Phosphatidylinositol 4-phosphate 5-kinase type I alpha |
| 배경 | Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha (PIP5K1A) is a ~62 kDa protein. Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility. PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis. Can also use phosphatidylinositol (PtdIns) as substrate in vitro. Together with PIP5K1C, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps. 1. Shulga, YV. et al. (2011) Journal of molecular biology 409, 101-12. PMID: 21477596 2. Shulga, YV. et al. (2012) The Journal of biological chemistry 287, 35953-63. PMID: 22942276 3. Loijens, JC. et al. (1996) The Journal of biological chemistry 271, 32937-43. PMID: 8955136 4. Xie, Z. et al. (2009) Molecular biology of the cell 20, 1695-704. PMID: 19158393 5. Chao, WT. et al. (2010) The Journal of cell biology 190, 247-62. PMID: 20660631 6. Mellman, DL. et al. (2008) Nature 451, 1013-7. PMID: 18288197 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human PIP5K1A Protein, N-His
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