中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HD558012 |
|---|---|
| 설명 |
Recombinant Human POLD4 Protein, N-GST & C-His (HD558012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9HCU8 |
| 단백질 길이 | Glu45-Leu107 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | DNA polymerase delta subunit 4, DNA polymerase delta subunit p12, POLD4, POLDS |
| 배경 | DNA polymerase delta subunit 4 is a ~12 kDa protein. As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb. Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites. 1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448 2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196 3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433 4. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200 5. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611 6. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480 |
| 참고사항 | For research use only |
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
