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| 카탈로그 번호 | HP842012 |
|---|---|
| 설명 |
Recombinant Human PRPF8 Protein, N-His (HP842012) expressed in E. coli, spanning Thr2071-Ala2335. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q6P2Q9 |
| 단백질 길이 | Thr2071-Ala2335 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 32.80 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | 220 kDa U5 snRNP-specific protein, p220, PRPF8, Splicing factor Prp8, PRP8 homolog, Pre-mRNA-processing-splicing factor 8, PRPC8 |
| 배경 | Pre-mRNA-processing-splicing factor 8 (PRPF8) is a ~273 kDa protein. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. 1. Luo, HR. et al. (1999) RNA (New York, N.Y.) 5, 893-908. PMID: 10411133 2. Schneider, C. et al. (2002) Molecular and cellular biology 22, 3219-29. PMID: 11971955 3. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346 4. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770 5. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166 6. Zhan, X. et al. (2018) Science (New York, N.Y.) 359, 537-545. PMID: 29301961 7. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106 8. Haselbach, D. et al. (2018) Cell 172, 454-464.e11. PMID: 29361316 9. Zhan, X. et al. (2018) Cell research 28, 1129-1140. PMID: 30315277 10. Fica, SM. et al. (2019) Science (New York, N.Y.) 363, 710-714. PMID: 30705154 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human PRPF8 Protein, N-His
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