Anti-Human PRPF8 Polyclonal Antibody (HP842014)

Name: Anti-Human PRPF8 Polyclonal Antibody
Brand: abinScience
SKU: HP842014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP842014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PRPF8 (Thr2071-Ala2335).

Target

220 kDa U5 snRNP-specific protein, p220, PRPF8, Splicing factor Prp8, PRP8 homolog, Pre-mRNA-processing-splicing factor 8, PRPC8

Purification

Purified by antigen affinity column.

Accession

Q6P2Q9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pre-mRNA-processing-splicing factor 8 (PRPF8) is a ~273 kDa protein. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

1. Luo, HR. et al. (1999) RNA (New York, N.Y.) 5, 893-908. PMID: 10411133

2. Schneider, C. et al. (2002) Molecular and cellular biology 22, 3219-29. PMID: 11971955

3. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346

4. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770

5. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

6. Zhan, X. et al. (2018) Science (New York, N.Y.) 359, 537-545. PMID: 29301961

7. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106

8. Haselbach, D. et al. (2018) Cell 172, 454-464.e11. PMID: 29361316

9. Zhan, X. et al. (2018) Cell research 28, 1129-1140. PMID: 30315277

10. Fica, SM. et al. (2019) Science (New York, N.Y.) 363, 710-714. PMID: 30705154

Note

For research use only.

Images

References

Datasheet

Anti-Human PRPF8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human PRPF8 Polyclonal Antibody (HP842014)

Overview

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