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| 카탈로그 번호 | HW321022 |
|---|---|
| 설명 |
Recombinant Human PTGER3 Protein, N-His-KSI (HW321022) expressed in E. coli, spanning Lys2-Ala53. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P43115 |
| 단백질 길이 | Lys2-Ala53 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 21.26 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | PTGER3, PGE2-R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, Prostaglandin E2 receptor EP3 subtype, PGE receptor EP3 subtype |
| 배경 | Prostaglandin E2 receptor EP3 subtype (PTGER3) is a ~43 kDa protein. Receptor for prostaglandin E2 (PGE2). The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G(i) proteins, and to an elevation of intracellular calcium. Required for normal development of fever in response to pyrinogens, including IL1B, prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required for normal potentiation of platelet aggregation by prostaglandin E2, and thus plays a role in the regulation of blood coagulation. Required for increased HCO3(-) secretion in the duodenum in response to mucosal acidification, and thereby contributes to the protection of the mucosa against acid-induced ulceration. 1. Schmid, A. et al. (1995) European journal of biochemistry 228, 23-30. PMID: 7883006 2. An, S. et al. (1994) Biochemistry 33, 14496-502. PMID: 7981210 3. Yang, J. et al. (1994) Biochemical and biophysical research communications 198, 999-1006. PMID: 8117308 4. Kunapuli, SP. et al. (1994) The Biochemical journal 298 ( Pt 2), 263-7. PMID: 8135729 5. Adam, M. et al. (1994) FEBS letters 338, 170-4. PMID: 8307176 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human PTGER3 Protein, N-His-KSI
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