Human PTGER3 Recombinant Protein (N-His) (HW321012)

Name: Human PTGER3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW321012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW321012
Description	Recombinant Human PTGER3 Protein, N-His (HW321012) expressed in E. coli, spanning Asn217-Gln359. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P43115
Protein length	Asn217-Gln359
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.36 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PTGER3, PGE2-R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, Prostaglandin E2 receptor EP3 subtype, PGE receptor EP3 subtype
Background	Prostaglandin E2 receptor EP3 subtype (PTGER3) is a ~43 kDa protein. Receptor for prostaglandin E2 (PGE2). The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G(i) proteins, and to an elevation of intracellular calcium. Required for normal development of fever in response to pyrinogens, including IL1B, prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required for normal potentiation of platelet aggregation by prostaglandin E2, and thus plays a role in the regulation of blood coagulation. Required for increased HCO3(-) secretion in the duodenum in response to mucosal acidification, and thereby contributes to the protection of the mucosa against acid-induced ulceration. 1. Schmid, A. et al. (1995) European journal of biochemistry 228, 23-30. PMID: 7883006 2. An, S. et al. (1994) Biochemistry 33, 14496-502. PMID: 7981210 3. Yang, J. et al. (1994) Biochemical and biophysical research communications 198, 999-1006. PMID: 8117308 4. Kunapuli, SP. et al. (1994) The Biochemical journal 298 ( Pt 2), 263-7. PMID: 8135729 5. Adam, M. et al. (1994) FEBS letters 338, 170-4. PMID: 8307176
Note	For research use only.

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SDS-PAGE for Recombinant Human PTGER3 Protein, N-His

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Datasheet

Human PTGER3 Recombinant Protein (N-His).pdf

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Human PTGER3 Recombinant Protein (N-His) (HW321012)

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