Human PUM1 Recombinant Protein (N-His) (HD611012)

Name: Human PUM1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD611012
Price: 211 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

50ug
100ug
1mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HD611012
설명	Recombinant Human PUM1 Protein, N-His (HD611012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
발현 시스템	E. coli
등록번호	Q14671
단백질 길이	Ser830-Ile1186
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	HsPUM, KIAA0099, PUM1, PUMH1, Pumilio homolog 1, Pumilio-1
배경	Pumilio homolog 1 is a ~126 kDa protein. Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. 1. Gupta, YK. et al. (2008) Structure (London, England : 1993) 16, 549-57. PMID: 18328718 2. Lu, G. et al. (2011) Structure (London, England : 1993) 19, 361-7. PMID: 21397187 3. Filipovska, A. et al. (2011) Nature chemical biology 7, 425-7. PMID: 21572425 4. Dong, S. et al. (2011) The Journal of biological chemistry 286, 26732-42. PMID: 21653694 5. Van Etten, J. et al. (2012) The Journal of biological chemistry 287, 36370-83. PMID: 22955276 6. Galgano, A. et al. (2008) PloS one 3, e3164. PMID: 18776931 7. Kedde, M. et al. (2010) Nature cell biology 12, 1014-20. PMID: 20818387 8. Leibovich, L. et al. (2010) Silence 1, 17. PMID: 20860814 9. Miles, WO. et al. (2012) Genes & development 26, 356-68. PMID: 22345517
참고사항	For research use only

이미지

참고 문헌

데이터시트

Human PUM1 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Human PUM1 Recombinant Protein (N-His) (HD611012)

개요

이미지

참고 문헌

데이터시트

SDS

빠른 접근

문의하기