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| 카탈로그 번호 | HX852012 |
|---|---|
| 설명 |
Recombinant Human RBX1 Protein, N-GST & C-His (HX852012) expressed in E. coli, spanning Ala2-Arg46. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P62877 |
| 단백질 길이 | Ala2-Arg46 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 33.05 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | RING finger protein 75, RNF75, E3 ubiquitin-protein transferase RBX1, N-terminally processed, RBX1, E3 ubiquitin-protein ligase RBX1, Rbx1, ROC1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING-box protein 1, Regulator of cullins 1 |
| 배경 | E3 ubiquitin-protein ligase RBX1 is a ~12 kDa protein. E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex mediates ubiquitination of Pol II subunit POLR2A at 'Lys-1268', a critical TC-NER checkpoint. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 1. Ohta, T. et al. (1999) Molecular cell 3, 535-41. PMID: 10230407 2. Kamura, T. et al. (1999) Genes & development 13, 2928-33. PMID: 10579999 3. Zheng, N. et al. (2002) Nature 416, 703-9. PMID: 11961546 4. Zhang, DD. et al. (2005) The Journal of biological chemistry 280, 30091-9. PMID: 15983046 5. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110 6. Cen, B. et al. (2009) The Journal of biological chemistry 284, 5265-76. PMID: 19112177 7. Isobe, T. et al. (2009) The Journal of biological chemistry 284, 27766-27779. PMID: 19679664 8. Duda, DM. et al. (2012) Molecular cell 47, 371-82. PMID: 22748924 9. Beck, J. et al. (2013) Nature cell biology 15, 430-9. PMID: 23455478 10. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human RBX1 Protein, N-GST & C-His
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