Anti-Human RBX1 Polyclonal Antibody (HX852014)

Name: Anti-Human RBX1 Polyclonal Antibody
Brand: abinScience
SKU: HX852014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HX852014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RBX1 (Ala2-Arg46).

Target

RING finger protein 75, RNF75, E3 ubiquitin-protein transferase RBX1, N-terminally processed, RBX1, E3 ubiquitin-protein ligase RBX1, Rbx1, ROC1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING-box protein 1, Regulator of cullins 1

Purification

Purified by antigen affinity column.

Accession

P62877

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase RBX1 is a ~12 kDa protein. E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex mediates ubiquitination of Pol II subunit POLR2A at 'Lys-1268', a critical TC-NER checkpoint. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

1. Ohta, T. et al. (1999) Molecular cell 3, 535-41. PMID: 10230407

2. Kamura, T. et al. (1999) Genes & development 13, 2928-33. PMID: 10579999

3. Zheng, N. et al. (2002) Nature 416, 703-9. PMID: 11961546

4. Zhang, DD. et al. (2005) The Journal of biological chemistry 280, 30091-9. PMID: 15983046

5. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

6. Cen, B. et al. (2009) The Journal of biological chemistry 284, 5265-76. PMID: 19112177

7. Isobe, T. et al. (2009) The Journal of biological chemistry 284, 27766-27779. PMID: 19679664

8. Duda, DM. et al. (2012) Molecular cell 47, 371-82. PMID: 22748924

9. Beck, J. et al. (2013) Nature cell biology 15, 430-9. PMID: 23455478

10. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346

Note

For research use only.

Images

References

Datasheet

Anti-Human RBX1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human RBX1 Polyclonal Antibody (HX852014)

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