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| 카탈로그 번호 | HP056012 |
|---|---|
| 설명 |
Recombinant Human SAMHD1 Protein, N-His (HP056012) expressed in E. coli, spanning Asp41-Asp583. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9Y3Z3 |
| 단백질 길이 | Asp41-Asp583 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 65.18 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | SAM domain and HD domain-containing protein 1, DCIP, Monocyte protein 5, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, MOP5, hSAMHD1, dNTPase, Dendritic cell-derived IFNG-induced protein, MOP-5, SAMHD1 |
| 배경 | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 is a ~72 kDa protein. Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. Not able to restrict infection by HIV-2 virus; because restriction activity is counteracted by HIV-2 viral protein Vpx. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools. 1. Rice, GI. et al. (2009) Nature genetics 41, 829-32. PMID: 19525956 2. Laguette, N. et al. (2011) Nature 474, 654-7. PMID: 21613998 3. Hrecka, K. et al. (2011) Nature 474, 658-61. PMID: 21720370 4. Goldstone, DC. et al. (2011) Nature 480, 379-82. PMID: 22056990 5. White, TE. et al. (2013) Cell host & microbe 13, 441-51. PMID: 23601106 6. Cribier, A. et al. (2013) Cell reports 3, 1036-43. PMID: 23602554 7. Schwefel, D. et al. (2014) Nature 505, 234-8. PMID: 24336198 8. Tang, C. et al. (2015) The Journal of biological chemistry 290, 26352-9. PMID: 26294762 9. Arnold, LH. et al. (2015) PLoS pathogens 11, e1005194. PMID: 26431200 10. White, TE. et al. (2017) Human mutation 38, 658-668. PMID: 28229507 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human SAMHD1 Protein, N-His
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