Anti-Human SAMHD1 Polyclonal Antibody (HP056014)

Name: Anti-Human SAMHD1 Polyclonal Antibody
Brand: abinScience
SKU: HP056014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HP056014

Description

Anti-Human SAMHD1 Polyclonal Antibody (HP056014) is a rabbit polyclonal antibody detecting SAMHD1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SAMHD1 (Asp41-Asp583).

Target

SAM domain and HD domain-containing protein 1, DCIP, Monocyte protein 5, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, MOP5, hSAMHD1, dNTPase, Dendritic cell-derived IFNG-induced protein, MOP-5, SAMHD1

Purification

Purified by antigen affinity column.

Accession

Q9Y3Z3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 is a ~72 kDa protein. Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. Not able to restrict infection by HIV-2 virus; because restriction activity is counteracted by HIV-2 viral protein Vpx. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools.

1. Rice, GI. et al. (2009) Nature genetics 41, 829-32. PMID: 19525956

2. Laguette, N. et al. (2011) Nature 474, 654-7. PMID: 21613998

3. Hrecka, K. et al. (2011) Nature 474, 658-61. PMID: 21720370

4. Goldstone, DC. et al. (2011) Nature 480, 379-82. PMID: 22056990

5. White, TE. et al. (2013) Cell host & microbe 13, 441-51. PMID: 23601106

6. Cribier, A. et al. (2013) Cell reports 3, 1036-43. PMID: 23602554

7. Schwefel, D. et al. (2014) Nature 505, 234-8. PMID: 24336198

8. Tang, C. et al. (2015) The Journal of biological chemistry 290, 26352-9. PMID: 26294762

9. Arnold, LH. et al. (2015) PLoS pathogens 11, e1005194. PMID: 26431200

10. White, TE. et al. (2017) Human mutation 38, 658-668. PMID: 28229507

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human SAMHD1 polyclonal antibody at 1ug/mL on recombinant Human SAMHD1 (Catalog No. HP056012).

References

Datasheet

Anti-Human SAMHD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human SAMHD1 Polyclonal Antibody (HP056014)

Overview

Images

References

Datasheet

SDS

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