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| 카탈로그 번호 | HC027012 |
|---|---|
| 설명 |
Recombinant Human SLC25A1 Protein, N-GST & C-His (HC027012) expressed in E. coli. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P53007 |
| 단백질 길이 | Glu47-Arg85&Glu106-Gly128&Glu144-Gln182&Leu241-Gly278 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 44.78 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Tricarboxylate transport protein, mitochondrial; Citrate transport protein; CTP; Mitochondrial citrate carrier; CIC; Solute carrier family 25 member 1; Tricarboxylate carrier protein; SLC25A1; SLC20A3 |
| 배경 | Tricarboxylate transport protein, mitochondrial is a ~34 kDa protein. Mitochondrial electroneutral antiporter that exports citrate from the mitochondria into the cytosol in exchange for malate. Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis-aconitate and to a lesser extent trans-aconitate, maleate and succinate. Substrate exchange across the membrane occurs consecutively with one substrate being transported first, then dissociating from the substrate binding site before the second substrate binds for transport in the opposite direction. In the cytoplasm, citrate plays important roles in fatty acid and sterol synthesis, regulation of glycolysis, protein acetylation, and other physiopathological processes. 1. Chaouch, A. et al. (2014) Journal of neuromuscular diseases 1, 75-90. PMID: 26870663 2. Majd, H. et al. (2018) Biochimica et biophysica acta. Bioenergetics 1859, 1-7. PMID: 29031613 3. Pop, A. et al. (2018) Journal of inherited metabolic disease 41, 169-180. PMID: 29238895 4. Li, W. et al. (2025) The EMBO journal 44, 1641-1662. PMID: 39881208 5. Cimadamore-Werthein, C. et al. (2024) The EMBO journal 43, 3450-3465. PMID: 38937634 |
| 참고사항 | For research use only |
SDS-PAGE for Recombinant Human SLC25A1 Protein, N-GST & C-His
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