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| 카탈로그 번호 | HD297012 |
|---|---|
| 설명 |
Recombinant Human SPOUT1 Protein, N-His (HD297012) expressed in E. coli, spanning Thr74-Thr376. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q5T280 |
| 단백질 길이 | Thr74-Thr376 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 35.35 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | 28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1 |
| 배경 | 28S rRNA (uridine-N(3))-methyltransferase is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. 1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501 2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046 3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266 4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325 5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233 |
| 참고사항 | For research use only |
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