Human TGM2 Recombinant Protein (C-His) (HB247011)

Name: Human TGM2 Recombinant Protein (C-His)
Brand: abinScience
SKU: HB247011
Price: 298 USD
Availability: InStock

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50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HB247011
설명	Recombinant Human TGM2 Protein, C-His (HB247011) expressed in Mammalian Cells, spanning Ala2-Ala687. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	Mammalian cells
등록번호	P21980
단백질 길이	Ala2-Ala687
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	80.16 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	Protein-glutamine gamma-glutamyltransferase 2, 2.3.2.13, Erythrocyte transglutaminase, Heart G alpha(h), hhG alpha(h), Isopeptidase TGM2, 3.4.-.-, Protein G alpha(h), G(h), Protein-glutamine deamidase TGM2, 3.5.1.44, Protein-glutamine dopaminyltransferase TGM2, 2.3.1.-, Protein-glutamine histaminyltransferase TGM2, 2.3.1.-, Protein-glutamine noradrenalinyltransferase TGM2, 2.3.1.-, Protein-glutamine serotonyltransferase TGM2, 2.3.1.-, Tissue transglutaminase, tTG, tTgase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase II, TGase II, Transglutaminase-2, TG2, TGase-2, hTG2, TGM2
배경	Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a ~77 kDa protein. Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. 1. Kanchan, K. et al. (2013) The Biochemical journal 455, 261-72. PMID: 23941696 2. Jeong, EM. et al. (2020) International journal of molecular sciences 21. PMID: 31991788 3. Nemes, Z. et al. (1997) The Journal of biological chemistry 272, 20577-83. PMID: 9252372 4. Upchurch, HF. et al. (1991) Journal of cellular physiology 149, 375-82. PMID: 1683874 5. Lai, TS. et al. (2017) Amino acids 49, 501-515. PMID: 27270573 6. Hernandez-Fernaud, JR. et al. (2017) Nature communications 8, 14206. PMID: 28198360 7. Melino, G. et al. (1994) Molecular and cellular biology 14, 6584-96. PMID: 7935379 8. Hsieh, YF. et al. (2013) PloS one 8, e81516. PMID: 24349085
참고사항	For research use only.