Anti-Human CD37 Recombinant Antibody (HH1) (HY564107)

Name: Anti-Human CD37 Recombinant Antibody (HH1)
Brand: abinScience
SKU: HY564107
Price: 196 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	HY564107
Species reactivity	Human
Applications	ELISA, FCM, IF, WB
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	HH1
Clonality	Monoclonal
Target	CD37, Tspan-26, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P11049
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Leukocyte antigen CD37 (TSPAN26) is a ~31 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B. Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT. 1. Lapalombella, R. et al. (2012) Cancer cell 21, 694-708. PMID: 22624718 2. de Winde, CM. et al. (2016) The Journal of clinical investigation 126, 653-66. PMID: 26784544 3. Meyer-Wentrup, F. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 178, 154-62. PMID: 17182550
Note	For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human CD37/TSPAN26 Antibody (HH1).
Flow Cytometry

Flow-cytometry using anti-human CD37 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD37 monoclonal antibody (Catalog HY564107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD37/TSPAN26 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 50ng
Lane 2: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 30ng
Lane 3: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 20ng