Human TRIM21/RO52 Recombinant Protein (N-His) (HB748012)

Name: Human TRIM21/RO52 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB748012
Price: 211 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

50ug
100ug
1mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HB748012
설명	Recombinant Human TRIM21/RO52 Protein, N-His (HB748012) expressed in E. coli, spanning Val287-Tyr475. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	P19474
단백질 길이	Val287-Tyr475
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	23.78 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	E3 ubiquitin-protein ligase TRIM21, 2.3.2.27, 52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RING finger protein 81, RING-type E3 ubiquitin transferase TRIM21, Ro(SS-A), Sjoegren syndrome type A antigen, SS-A, Tripartite motif-containing protein 21, TRIM21, RNF81, RO52, SSA1
배경	E3 ubiquitin-protein ligase TRIM21 (RO52) is a ~54 kDa protein. E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. 1. Wada, K. et al. (2006) Biochemical and biophysical research communications 339, 415-21. PMID: 16297862 2. Wada, K. et al. (2006) Biochemical and biophysical research communications 339, 731-6. PMID: 16316627 3. Wada, K. et al. (2006) Biochemical and biophysical research communications 342, 253-8. PMID: 16472766 4. Sabile, A. et al. (2006) Molecular and cellular biology 26, 5994-6004. PMID: 16880511 5. Takahata, M. et al. (2008) Molecular immunology 45, 2045-54. PMID: 18022694 6. Yamochi, T. et al. (2008) Biochemical and biophysical research communications 370, 195-9. PMID: 18361920 7. Higgs, R. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 1780-6. PMID: 18641315 8. Espinosa, A. et al. (2008) Experimental cell research 314, 3605-13. PMID: 18845142 9. Wada, K. et al. (2009) Journal of biochemistry 146, 821-32. PMID: 19675099 10. Kimura, T. et al. (2015) The Journal of cell biology 210, 973-89. PMID: 26347139
참고사항	For research use only.